Effect of protein supplementation on some rumen parameters in cattle
Keywords:
protein supplementation, soya bean meal, ruminal parametersAbstract
The objective of this study was to measure the effect of soya bean supplementation on some ruminal parameters. A diet composed of rye grass hay was given to Holstein-Freisian dry cows fitted with permanent rumen fistulae, with (CS) or without (SS) supplementation of 1 Kg soya bean meal per cow day. Samples of rumen content were withdrawn at 0, 2,4, 6,8; and 10 hours after supplementation, simultaneously for both treatments. The samples were processed for: ammonia, ATP and volatile fatty acids (AGV) concentration, AGV composition and rumen bacterial counts. Significant differences between treatments were found for ammonia concentration (CS: 86.4 mg/l; ss. 53.7 mg/I; P<0.01), number of bacteria (CS: 1.11 x 109; SS: 0.68x 109; P<0.01) and propionic acid concentration (CS: 16.8 mmol/I; SS: 15.4 mmol/I; P<0.01) and also between sampling times for ATI' concentration, in both treatments, when zero hour (sample taken immediately before supplementation) was compared with 2, 4 and 6 hours (P<0.01): zero hour: 9.7 g ATP/g; two hours: 20.6 g/g; four hours: 19.7 g/g; six hours: 25.7 g/g. The number of rumen microbes was 62% higher in CS when compared to SS, indicating the effect of soya bean meal upon rumen population growth.
Downloads
References
BARRY, T.N. Y JOHNSTONF. P.D. 1976. A comparison al supplementary sources of nitrogen and energy for increasing the voluntary intake and utilizalion of barley straw by sheep. J. agric. Sci. (Camb.) 86: 163-169.
CAMPBELL, L.O.; EGGUM, B.O. Y WOLSTRUP, J. 1982. Influcnce of dietary antibiotic on nitrogen, ami no acid and energy digestion by rats and adult roosters, Can. J. Anim. Sci. .62: 537-545.
CAMPLlNG, R.C.; FREER. M. y BALCH, c.e 1962. Factors inffecting the voluntary intake of food by cows. 3. The effect of urea on the voluntary inlake of oal straw. Br, J. Nutr. 16: 115-124.
CONW AY, E.J. Y O'MALLEY, E 1942. Microdiffusion methods. Arnmonia and urea using buffered absorbents. Biochem. J. 36: 655-001.
COOMBE. J.a y TRlBE, O.E. 1963. The effects of urea supplement.; on the ulilization of straw
plus molasses diets by sheep. Ausl. J. Agric. Res. 14: 70-91.
COTIRILL, B.R.; BEEVER. O.E.; AUSTIN, A.R. Y OSBOURN, D.F. 1982. The effect of protein and non - protein - nitragen supplements lo maize silage on total amino acid supply in young cattle. Br. J. Nutr. 48: 527-541.
EGAN, J.K. y OOYLF. P.T. 1985. Effect of intraruminal infusion of urea on the response in vohmlary food intake by sheep, Ausl. J. Agric. Res. 36: 483-495.
EGGUM, B.O.; BEAMES, R.M.; WOLSTRUP, J. Y BACH KNUDSEN, K.E. 1934. The effect of prolein quality and fibre level in the diel and microbial adiv;ly in the digestivo tract on protein utilization and energy digestibility in rats, Br, J. Nutr. 51: 305-314.
ERFLE, J.O.; MAHAOEVAN, O. Y SAUER, F.O. 1979. Effecl of diet quality on adenosine _ 5'- triphospMte c:oncentnlion and adenylale charge of rumen microbes from fistulated cows. J. Oairy Sci. 62: 2M-291.
FORBES, J.M. i986. The voluntary intalce of farm animals. Butterworlhs, U.K., 206 pp.
GILL, M. Y ENGLAND, P. 1984. Effect of degradability of protein supplernents on voluntary intake and nitrogen retention in young catUe fed grass silage. A.•.•im, Prod, 3~ 3t-36.
GILL, M.; BEEVER. O.E.; BlTITERY, P.].; ENGLAND, P.; GIBB, M.J. ':i BAKE...T{, RD. 1957. The effect of oestradiol ~ 178 implantation on the response in voluntary intake, iive _weight gain and body c:orilpo6ition to fishmeal supplemenlation of silage offered lo growing calves. J. Agric. Sci. {Camb.) 108: 9-16.
GRUMMER. R.R.; CLARCK. J.H.; DA VIS, C.L. Y MURPHY, M.R. i984. Effect of ruminal ammonia - nitrogen concenlralion on protein degradation in situ. J. Dairy Sci. 67: 2294-2301.
HA, ].K.; KENNELLY, J.J. y BERZINIS, R. 1986. Eñect ofdietary nitrogen SOUF-~0: microbíal protein .ynlhesis, dielary protein degradatíon and nutrient digestion in steers, Anim, Feed Sci. TechnoI. 14: 117-126.
HARRISON, D.G. Y MCALLAN, A.B. 1980. Factors lIffecting microbial growth vields in lhe reticulo - rumen.ln: digestive physiology and metabolism in rurninants (R'¡c~busch, Y. y Thivend, P. eds.], MTP. U.K., pp 205-226.
HORTON, G.M.J. Y NICHOLSON, H.H. 19&1.Nilrcgcn sources ter growing caltle.fed barley and either wheat straw cr dehydrated ~:C;)iía.J. Anim. SO. 52: ¡14;;'1149.
HUNGATF., R.E. 1969. A rol: - tube method for cullivalion of striet anaeraba. Methods Microbio!. 38: ¡¡1-¡52.
HUQUE. Q.M.E. y TIiOMSEN, K.V. 1984. Source of nitrogen for romen núcrobes. Act. Agric. Scand, 34: 26-:32.
KELLAWAY, R.e. y LEIEHOLZ, L 1983. Effects of r.itrogen supplements on intake and utilizalíon of low - 'iua1ity forages, World Anim. Rev, 48: 3;;'37. .
KROPP, J.R.; JOHNSON, R.K: MALES, J.R Y OWENS, F.N. 1977. Mkrobial protein synlhesis wilh low qualily roughage rations: Isoniirogenous substitution of urea foc soybenmeal. J. Anim. Sci. 46: 837-343.
LEE. G.J.; HEf.'NESSY, D.IN.; NOL .••.N, ¡.V. y LENG, R.A. 1937. Response to nitrogen and maize aupplements by young cattle offered a low quality pasture hay. Aust. J. Agric. Res. 38: 195-207.
LOERCH, s.c, BERGER,. L.L; GIANOLA, O. y FAHEY, G.C. 1983. Effects of dietary protein source and energy level on in si tu nitrogen disapper.uance of various protein sounes. J. AnÍln. Sci. 56: 206-216.
MAENG, W.J.; VAN NEVEL, c.t, BALDWIN, R.L. Y MORRIS, J.G. 1916. Rumen microbial
growth rates and yields: Effects of arnino acids and protein. J. Dairy Sci. 59: 63-79.
MCAlLAN, A.B. y SMlTI!. R.H. 1983. Factcrs inih..encing lhe digestion al dietary carbohydrates between the moulh and abomasurn of steers. B~.J. Nutr, 50: 445-454.
MCMANUS, W.R.; MANTA. L.; MCFARLMiE. J.D. y GRAY, A.C. 1972. The effects of diet supplements and gamma irradiation on dissimilation of low - quality rou¿:hages by ruminants. 1. Studies on the terylene . bag techniqua and effects of supplementation on base ration. J. Agric. Sci. (Camb.) 79: 27-40.
MEHREZ. A.z.; ORSKOV, E.R. Y MCDONALO, 1. 1977. Rales of rumen fermentation in relation lo ammonia concentration. Br. J. Nutr. 38: 437443.
OLOHAM, J.O.; Btrr'IERY, P.J.; SWAJIJ, H. y lEWIS, O. 1977. Interactions between dietary carbohydrate and nitrogen and digestion in sheep, J. Agric, Sci. (Camb.) 11.9:~7-479.
ORSKOV, E.R.; FRASER, C. Y MCDONALD, I. 1971. Digestion of concentra tes in sheep. 2. The effect of urea oe fishmeal supplementation of barley diets on the apparent digestion al protein, fat, stareh anJ ash in the rumen, the small intestine and the large intestine and calculation of volatile ecid p~oduclion. Br.], Nutr, 25: 24;;'251.
ROOKE. O.N. y ARMSTRONG, O.G. 1987. The digestion by cattle of silage and barley diets ecntaining increasing quantities of fishmeal, J. Agric, Sci. (Camb.) 109: 261-272.
SALn:R, O.N.; [)ANESHVAR., K. y SMITH, R.H. 1979. The origin of N incorporated into compounds in the rumen bacteria of steers given protein - Ilnd urea - containing dieta. Dr.J. Nulr.41: 191-209. .
SAS. 1985. SAS User'. Cuide: Statislics. SAS Jnst.•Cary, Noa1h Carolinil, U.S.A., 5M pp. SA'ITER. LD. Y ROFFLER, R.F- 1976. ReLationship between ruminaJ ammonia and non - protein niltogen utilization by ruminanls. In: Traeer studies on t.on - protein nitrogen for rumínanla DI, Viena, Austria, pp. 119-138.
SMITII, T; IlROS'ffiR. V.J. y HILL, R.E. 1980. A comparíson of supplementary nitrogen for young cattle receiving hbre - rieh dieta. J. Agrie. Sci. (Camb.) 95: 687-695.
SivilTIl, T.; GRJGERA-NAON, J.J. y BROSTER. W.H. 1983. Nilrogen ti:;,! in diels eontaining
procased .traw. IV Inl Symp. Protein Metabolísm and Nutrition, Les Colloques de
'lnn, 16, pp. 1.5-148.
SMITH, T.; S1VTrnR. J.W. y MERRY, R.J. 1985. Further comparisons of energy and protein 1OUra:3loc growing cattle, J. Agríe, Sci. (Camb.) 104: 485-481.
STRITZLER. N.P. 1988. The effect ol nilrogen and energy supplementation on the digestion of non - stareh polysaeeharides and mierobial activity in the rumen of eows fed fibre - rich diets, Ph. D. Thesis, The Royal Vet. Agric. Univ., Copenhague, Dinamarca, 2i9 pp.
STRJTZLER. N.P. 1990. Efecto de la suplementación con harina de soja sobre algunas parámetros ruminales (resumen). Rev. Arg. Prod. ARim. 10 (supl. 1): 9.
STRJTZLER. N.P.; GALLARDO, M. Y GINGINS, M.A. 1983. Suplementación nitrogenada en fcxn¡es de baja calidad. Rev. Arg. Prod, Anim. 3: 283-309.
rnOMSEN, K.V. 1985. The specifie nitrogen requirements ol rumen mieroorganisms. Acta Agríe. Sand. 37: .37"""8.
lHOMSEN, K.V. Y JOHNSEN,F. 1984. Avalability of nilrogen in different protein soun:es, padicularlyas regards fish visoera silage for in vitro digestibility ol stan:h and cellulose. Acta Agrie. Scand. 34: 17-25.
VARVII
VIK-MO, L Y LINDBERG, J.E. 1985. In sacco degradability al prolein (N) and dry matter in samples of individual leeds oc eombinations tested with diets medium oc high in pnMin. Acta Agrie. Scand. 35: 117-128.
WESTON, R.H. 1967. Factors limiling the intake of leed by sheep. n. Studies wi!h wheaten hay. Aust. J. Agrie. Res. 18: 983-1002-
WOLSTRUP, J. Y JENSEN, K. 1976. Adenosine lriphosphate in the bovine rumen during' DWámum nutrient supply and starvation. J. Appl. Bacteriol. 41: W-2S0.
WOLSTRUP, J. Y JENSEN, K. 1978. Adenosine lriphosphate and desoxiribonudeie acid in the alimentary tract ol cattle fed different nitrogen sourees. J. Appl. Bacteriol. 45: 49-56.
Downloads
Published
Issue
Section
License
La Editorial de la Universidad Nacional de La Pampa (EdUNLPam) exigirá a los/as autores/as la firma del siguiente documento:
La EdUNLPam lleva a cabo la publicación del artículo: (Título del Artículo) en SEMIÁRIDA Rev.Fac.Agron UNLPam ISSN 2362-4337 (impresa) ISSN 2408-4077 (en línea), del cual el/los abajo firmantes son autores de una o más partes. En el mismo acto, el/los autores entregan exclusivamente a la EdUNLPam todos sus derechos protegidos por las leyes de propiedad intelectual que rigen en la Argentina para reproducir, publicar, editar, fijar, comunicar y transmitir públicamente en cualquier formato o medio impreso o electrónico, inclusive internet, el artículo enviado a publicación e incluirlo en índices o bases de datos nacionales e internacionales. A cambio, la EdUNLPam entrega a los autores la autorización para la publicación o reimpresión con ines académicos y educativos en cualquier libro o medio de divulgación, con la sola obligación de citar el artículo original publicado en la EdUNLPam. Cada autor acuerda en que el material provisto a la EdUNLPam es un trabajo original, que no ha sido impreso o publicado en cualquier otro medio con anterioridad y que no vulnera derechos de terceros. El Primer autor tendrá la posibilidad de leer y corregir el artículo ya editado como “prueba de galera”, pero si el autor no devolviera esas correcciones de la prueba de galera dentro del tiempo especificado, el proceso de producción y publicación podrá proseguir sin la aprobación del autor. El/los autor/es no recibirán compensación monetaria de la EdUNLPam por el uso del material contenido en este artículo y asumen la responsabilidad de las opiniones vertidas en él.
.png)
22.png)
.jpg)
.jpg)
