Guide for the use of immunodiagnostic in sheep applied to the epidemiological surveillance of cystic echinococcosis

  • Verónica Thelma Poggio Instituto de Ciencia y Tecnología César Milstein (CONICET), Buenos Aires, Argentina
  • Guillermo Bernanrdo Mujica Ministerio de Salud. Rio Negro. República Argentina
  • Joaquin Prada Faculty of Health and MedicalSciences, University of Surrey,Guildford, UK
  • Edmundo Juan Larrieu Instituto de Zoonosis. Universidad Nacional de La Pampa

DOI:

https://doi.org/10.19137/cienvet202224207

Keywords:

Echinococcosis, Immunodiagnostics, Sheep, Surveillance, Control

Abstract

Cystic echinococcosis (CE) is a parasitic zoonosis caused by Echinococcus granulosus sensu lato. Immunodiagnostic techniques such as Western blot (WB) or enzyme-linked immunosorbent assay (ELISA), with different antigens, can be applied to the diagnosis of CE sheep for epidemiological surveillance purposes in control programs. However, its use is limited by the existence of antigenic cross-reactivity between different species of taeniidae present in sheep. Despite this, the usefulness of establishing surveillance systems based on the identification of infection present in a livestock establishment, known as the Epidemiological Unit (UE), has been postulated. A new ELISA diagnostic technique has been developed and validated using the recombinant EgAgB8/2 antigen for the detection of antibodies against E. granulosus, estimating an OD 0.496 as the cut-off value that optimizes diagnostic sensitivity and specificity. To determine the infection in the EU, a Bayesian model was built, executed with a Monte Carlo Markov chain algorithm, which allowed defining the sample sizes for different expected prevalences to ensure that at least two of the samples are truly positive. In this way, the system can be used to identify the prevalence of infection in the area under control measured as a percentage of EU with the presence of infected sheep (infection present) or individually identify the EU with present transmission, given by presence of infected lambs, on which control measures should be intensified.

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Author Biographies

Verónica Thelma Poggio, Instituto de Ciencia y Tecnología César Milstein (CONICET), Buenos Aires, Argentina

Investigador CONICET

Guillermo Bernanrdo Mujica, Ministerio de Salud. Rio Negro. República Argentina

Salud Ambiental Bariloche, Ministerio de Salud, Provincia de Río Negro, Viedma, Argentina

Joaquin Prada, Faculty of Health and MedicalSciences, University of Surrey,Guildford, UK

Faculty of Health and MedicalSciences, University of Surrey,Guildford, UK

Edmundo Juan Larrieu, Instituto de Zoonosis. Universidad Nacional de La Pampa

Director Instituto de Zoonosis. Facultad de Ciencias Veterinarias. UNLPam. Profesor Consulto, Facultad de Ciencias Veterinarias. UNLPam

References

1. Thompson RCA, McManus D. Aetiology: parasites and life cycles. In: Eckert J, Gemmel M, Meslin F, Pawlowsk,i Z. WHO/OIE Manual on Echinococcosis in Humans and Animals: a public health problem of global concern. WHO/OIE. France. 2001; p. 1-10.
2. Craig PS, Hegglin D, Lightowlers MW, Torgerson PR, Wang Q. Echinococcosis: Control and Prevention. In: Thompson, R.C.A., Deplazes, P., Lymbery, A. J., (Eds.), Echinococcus and Echinococcosis, 2017; Part B, pp. 55–158.
3. Moro P, Verastegui M, Gilman RH, Falcon N, Bernal T, et al. Enzyme-linked immunotransferblot assay for diagnosis of hydatidosis (Echinococcus granulosus) in sheep. Vet. Rec. 1997; 140, 605–606.
4. Cabrera PA, Irabedra P, Orlando D, Rista L, Harán G, et al. National prevalence of larval echinococcosis in sheep in slaughtering plants Ovis aries as an indicator in control programmes in Uruguay. Acta Trop. 2003; 85(2):281-5.
5. Craig P, Mastin A, van Kesteren F, Boufana B. Echinococcus granulosus: epidemiology and state-of-the-art of diagnostics in animals. Vet. Parasitol. 2015; 213:132e148.
6. McManus DP. Immunodiagnosis of sheep infections with Echinococcus granulosus: in 35 years where have we come? Parasite Immunol. 2014; 36(3):125-30.
7. Gatti A, Alvarez AR, Araya D, Mancini S, Herrero E, et al. Ovine echinococcosis. I. Immunological diagnosis by enzymeimmunoassay. Vet. Parasitol. 2007; 143:112–121.
8. Guarnera EA, Santillan G, Botinelli R, Franco A. Canine echinococcosis: an alternative for surveillance epidemiology. Vet Parasitol. 2000; 88(1-2):131-4.
9. Lamberti R, Cavagion L, Gatti A, Calvo C, Gino L, Puches VV, et al. Humoral response and evolution of Echinococcus infection in experimentally infected sheep. Rev. Bras. Parasitol. Vet. 2014; 23(2),237-40.
10. Larrieu E, Alvarez AR, Gatti A, Mancini S, Bigatti R, et al. Fisiopatología y respuesta inmune de ovinos experimentalmente infectados con Echinococcus granulosus. Medicina (B. Aires). 2009; 69(3),341-346.
11. Sykes AI, Larrieu E, Poggio TV, Céspedes G, Mujica G, et al. Modelling diagnostics for Echinococcus granulosus surveillance in sheep using Latent Class Analysis: Argentina as a case study. One Health. 2021; 4,14:100359
12. Poggio TV , Gómez JM , Boado LA , Vojnov AA, Larrieu E, Mujica GB, et al. Immunodiagnosis of cystic echinococcosis in livestock: Development and validation dataset of an ELISA test using a recombinant B8/2 subunit of Echinococcus granulosus sensu lato. Data in Brief 42 (2022) 108255.
13. Larrieu E. Manual de Epidemiologia y Salud Pública Veterinaria. Editorial UNRN, Rio Negro, Argentina. 2021; 181pp.
Imagen ilustrativa

Published

2022-07-26

How to Cite

Poggio, V. T. ., Mujica, G. B. ., Prada, J., & Larrieu, E. J. (2022). Guide for the use of immunodiagnostic in sheep applied to the epidemiological surveillance of cystic echinococcosis. Ciencia Veterinaria, 24(2), 224–233. https://doi.org/10.19137/cienvet202224207

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Section

Comunicaciones