Polymorphisms of BoLA-DRB3 gene and its association with resistance / susceptibility to Leucosis in Holstein cattle from La Pampa

  • Laura Rosana Baltian UNLPam. Facultad de Ciencias Veterinarias
  • Ana Valeria Follmer UNLPam. Facultad de Cs. Veterinarias.
  • Delia Lidia Peratta UNLPam. Facultad de Ciencias Veterinarias
  • Enrique Eberardo Schmidt UNLPam. Facultad de Cs. Veterinarias Ministerio de la Producción. Gobierno de La Pampa
  • Rocío Antonella Severini UNLPam. Facultad de Cs. Veterinarias
  • Cristian Borrego UNLPam. Facultad de Cs. Veterinarias
  • Nicolás José María Alvarez Rubianes UNLPam. Facultad de Ciencias Veterinarias
  • María Verónica Rípoli 2IGEVET- CONICET, FCV- UNLP
  • Guillermo Giovambattista IGEVET- CONICET, FCV- UNLP

Keywords:

BoLA-DRB3 gene, PCR-RFLP, resistance, susceptibility, leucosis

Abstract

EBL is a disease of adult cattle caused by the retrovirus, bovine leukemia. It can manifest: aleukemic an asymptomatic form, with a normal number of B lymphocytes in the blood; a form of permanent lymphocytosis, with an increase in the number of B lymphocytes and finally a lymphoproliferative tumor presenta­tion in the form of lymphosarcoma. The alleles of the genes of the Bovine Major Histocompatibility Complex (BoLA) have been associated with resistance and susceptibility to infectious dis­eases. The overall objective of this study was to associate poly­morphisms of the BoLA-DRB3.2 gene, defined by the technique of polymerase chain reaction technique and polymorphisms length of the restriction fragment (PCR-RFLP), with resistance / susceptibility to leucosis in Holstein cows of La Pampa. It relied on a case/control design, for which blood samples were taken to 150 animals in 3 opportunities with intervals of three months each. The case group comprised animals that were positive in the immunodiffusion agar animals test (DIDA) and whose blood lymphocyte count was ≥ 10.000 linf/μl and the control group included cows that ware negative for DIDA and present < 10.000 linf/μl of blood. Fisher’s Exact test and Odds Ratio (OR) of Woolf-Haldane were used to study the association between lym­phocyte count and DIDA results with allelic variants. In 82 ani­mals genotyped by PCR-RFLP, the DRB3.2*22 allele showed an OR = 5.332 (p = 0.0138) and DRB3.2*11 allele had a OR value of 0.11 (p=0.001) and were the most frequent in the control group and in the case group, respectively. These results would showed DRB3.2*22 allele and DRB3.2*11 allele a high risk of having leucosis (susceptibility) and low risk to suffer (resistance) re­spectively. The analysis of the relationship between alleles of the BoLA and resistance/susceptibility to leucosis could be im­proved with deep research on family lineages of dairy cows.

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Author Biographies

Laura Rosana Baltian, UNLPam. Facultad de Ciencias Veterinarias

Técnicas de manipulación del ADN, como extracción, Reacción en Cadena de la Polimerasa (PCR),Polimorfismos de la longitud de los fragmentos de restricción (RFLP), Las mismas se usan en investigación y docencia. Como asi también en el servicio que ofrece el lab de genética para estudios forenses. La secuenciación de ADN se realza en el IGEVET, UNLP- CONICET.

Ana Valeria Follmer, UNLPam. Facultad de Cs. Veterinarias.

Becario de Grado

Delia Lidia Peratta, UNLPam. Facultad de Ciencias Veterinarias

Médica Veterinaria, docente de las cátedras Producción de Bovinos de Carne y de Genética y Mejoramiento Animal, con especialidad en Docencia Universitaria en Ciencias Veterinarias y en Higiene y Seguridad en el Trabajo Agrario, específicamente desde el bienestar animal

Rocío Antonella Severini, UNLPam. Facultad de Cs. Veterinarias

Becario de grado.

Cristian Borrego, UNLPam. Facultad de Cs. Veterinarias

Becario de grado.

María Verónica Rípoli, 2IGEVET- CONICET, FCV- UNLP

Licenciada en Biología (FCNyM, UNLP, año 1995), Doctora en Ciencias Naturales (FCNyM, UNLP, año 2001), Magister en Biología Molecular e Ingeniería Genética (Universidad Dr. René Favaloro, año 2001), Investigadora Adjunta de la Carrera de Investigador del Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Lugar de trabajo: IGEVET (Instituto de Genética Veterinaria-UNLP-Conicet La Plata).

Published

2017-08-08

How to Cite

Baltian, L. R., Follmer, A. V., Peratta, D. L., Schmidt, E. E., Severini, R. A., Borrego, C., … Giovambattista, G. (2017). Polymorphisms of BoLA-DRB3 gene and its association with resistance / susceptibility to Leucosis in Holstein cattle from La Pampa. Ciencia Veterinaria, 18(1), 9–27. Retrieved from https://cerac.unlpam.edu.ar/index.php/veterinaria/article/view/1895

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Section

Artículos de Investigación