Estudio de asociación del polimorfismo del gen de clase II BoLA-DQA1 con resistencia/ susceptibilidad a mastitis en ganado Holstein de la provincia de La Pampa

  • Laura Rosana Baltian Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias
  • María Verónica Ripoli Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias
  • Guillermo Giovambattista Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias
Palabras clave: BoLA-DQA1, resistencia, susceptibilidad, mastitis, células somáticas

Resumen

La mastitis es una enfermedad frecuente en el ganado lechero que conduce a una disminución en la producción y a un incremento en los
costos sanitarios. Esta enfermedad puede evaluarse a través del número de glóbulos blancos por mililitro de leche, conocido como conteo de células somáticas (CCS). La variabilidad alélica de los genes del Complejo Principal de Histocompatibilidad Bovino (Bovine Leukocyte Antigen,
BoLA) se ha asociado con resistencia y susceptibilidad a enfermedades infecciosas. El objetivo del presente trabajo fue estudiar el polimorfismo del gen de clase II BoLA-DQA1 y su asociación con resistencia /susceptibilidad a mastitis medida a través del CCS en leche de ganado Holstein de la provincia de La Pampa. La población se dividió en: 1) grupo caso, con alto CCS y presencia de mastitis (≥ 250.000 cel/ml, susceptible) y 2) grupo control, con bajo CCS (< 250.000 cel/ml, resistente). El BoLADQA1 se genotipo en 60 animales mediante PCR-RFLP y PCR-SBT. El test exacto de Fisher y Odds Ratio (OR) de Woolf-Haldane se utilizaron para estudiar la asociación entre el CCS y las variantes alélicas. Se detectaron 16 alelos del gen BoLA-DQA1 y el alelo BoLA-DQA1*0101 evidenció un OR de 4 (p = 0,058). Se destaca la importancia de los alelos del BoLADQA1 para seleccionar animales resistentes a enfermedades.

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Biografía del autor/a

Laura Rosana Baltian, Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias

Formación: Lic. En Biología (orientación Zoología). FCN y M, UNLP Especialista en Docencia Universitaria en Ciencias Veterinarias. FCV, UNLPam Dra. En Ciencias Veterinarias. FCV, UNLP.
Actividad Académica: Profesora Adjunta. Regular. Cátedra de Genética y Mejoramiento Animal. FCV, UNLPam

María Verónica Ripoli, Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias

Formación: Lic. en Biología (orientación Zoología). FCN y M, UNLP Dra. en Ciencias Naturales. FCN y M, UNLP
Actividad Académica

Guillermo Giovambattista, Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias

Formación: Lic. en Biología (orientación Zoología). FCN y M, UNLP
Dr. en Ciencias Naturales. FCN y M, UNLP
Actividad Académica. Profesor Adjunto regular. Cátedras de Genética general y de Genética Forense Veterinaria. FCV, UNLP. 
Investigador Principal del CONICET.

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Publicado
2022-03-07
Cómo citar
Baltian, L. R., Ripoli, M. V., & Giovambattista, G. (2022). Estudio de asociación del polimorfismo del gen de clase II BoLA-DQA1 con resistencia/ susceptibilidad a mastitis en ganado Holstein de la provincia de La Pampa. Ciencia Veterinaria, 24(1), 1-18. https://doi.org/10.19137/cienvet202224101
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