Efecto del ácido alfa-lipoico en microsomas de cerebro y corazón de rata sometidos a estrés oxidativo inducido por ascorbato-Fe++

Mariana Beatriz Gavazza; Monica Marmunti; Alejandro Palacios

doi:10.19137/cienvet202426104

Mariana Beatriz Gavazza Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias https://orcid.org/
Monica Marmunti Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias.
Alejandro Palacios Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias.

DOI:

https://doi.org/10.19137/cienvet202426104

Keywords:

Alpha-lipoic acid, Oxidative stress, Microsomes, Brain, Heart

Abstract

Oxidative stress is caused by the overproduction of reactive oxygen species that generate an imbalance in the cellular antioxidant capacity. In this study, the effect of alpha-lipoic acid on microsomes isolated from rat brain and heart was analyzed by determining chemiluminescence (expressed as counts per minute) and thiobarbituric acid reactive substances. Oxidative stress was induced by subjecting the samples to an ascorbate-Fe++-dependent pro-oxidant system at 37 °C for 120 minutes. The inhibitory effect of alpha-lipoic acid was compared using different concentrations thereof, corresponding to 50, 150 and 250 µg per mg of microsomal protein. Controls were performed simultaneously without the addition of the prooxidant. A significant increase in light emission and malondialdehyde was observed in the microsomes of both organs in the ascorbate-Fe++ group. Analysis of chemiluminescence levels and thiobarbituric acid reactive substances indicated that alpha-lipoic acid acted as an antioxidant that protected rat heart microsomes from damage by lipid peroxidation at all doses tested. In brain microsomes, alpha-lipoic acid was observed to act as an antioxidant only at the 150 µg/ml dose. In the latter case, it will be necessary to test new doses of it to demonstrate the effects on these membranes. In conclusion, alpha-lipoic acid acted as an antioxidant to protect the membranes of both organs against peroxidative damage

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Author Biographies

Mariana Beatriz Gavazza, Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias

Licenciada en Biología (Orientación: Zoología), Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata. Doctora en Ciencias Naturales, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata. Realizo docencia e investigación en la Cátedra de Bioquímica, FCV, UNLP. Integrante del proyecto de investigación: 2019 Código 11/V276 Principios activos de origen animal y vegetal efectos sobre las membranas biológicas. Director: Alejandro Palacios. Institución otorgante: Universidad Nacional de La Plata; Secretaría de Ciencia y Tecnología

Monica Marmunti, Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias.

Licenciada en Biología (Orientación: Ecología), Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata. Doctora en Ciencias Naturales, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata. Realizo docencia e investigación en la Cátedra de Bioquímica, FCV, UNLP. Integrante del proyecto de investigación: 2019 Código 11/V276 Principios activos de origen animal y vegetal efectos sobre las membranas biológicas. Director: Alejandro Palacios. Institución otorgante: Universidad Nacional de La Plata; Secretaría de Ciencia y Tecnología

Alejandro Palacios, Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias.

Médico Veterinario, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata. De Post-Grado: Doctor en Ciencias Veterinarias, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata. Realizo docencia en las Cátedras de Bioquímica (Profesor Titular dedicación exclusiva Ordinario) y Fisiología (Jefe de trabajos Prácticos dedicación simple Ordinario) e investigación en el Laboratorio de Bioquímica, FCV, UNLP. Director del proyecto de investigación (2019-2023) Código 11/V276 Principios activos de origen animal y vegetal efectos sobre las membranas biológicas. Institución otorgante: Universidad Nacional de La Plata; Secretaría de Ciencia y Tecnología.

Miembro del Consejo Directivo, representante del Claustro de Profesores.

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Funding This work was supported by the Secretariat of Science and Technology, National University of La Plata (Project 11/V276).
Credits Gavazza M, Marmunti M, Palacios: the three authors worked on conceptualization, validation, formal analysis, research, writing, review, editing and visualization. GM and PA worked on methodology and writing -draft original. PA worked on resources, data curation, supervision and project administration.
Conflict of interest statement
The authors declare that, during the research process, there has been no type of personal, professional or economic interest that could have influenced the judgment and/or actions of the researchers at the time of preparing and publishing this article.