Determination of the Toxic Effect of Pulmonary Extracts on Refrigerated Porcine Semen

  • Dante Alberto Cerutti Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias http://orcid.org/0000-0003-2748-3578
  • Melina Castillo Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias http://orcid.org/0000-0002-4738-7543
  • Sebastian Ramos Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias.
  • Mirta Adriana Koncurat Universidad Nacional de La Pampa. Facultad de Ciencias Veternarias
  • Humberto Cisale Universidad de Buenos Aires

DOI:

https://doi.org/10.19137/cienvet202123103

Keywords:

porcine semen, pulmonary surfactants, cryopreservation

Abstract

Synthetic surfactants have shown positive effects upon the integrity of the cell membrane of cryopreserved sperm without affecting their motility. As pulmonary extracts have surfactant substances, they are expected to produce a positive effect on the boar sperm fertility after cryopreservation. In order to have preliminary evidence regarding the absence / presence of toxicity of pulmonary extracts on semen, ejaculates from two boars, aged between 24 and 30 months, were used. Each ejaculate was divided into two groups. One of them was diluted with a middle-term commercial medium (Control group) and the other with the same medium plus the addition of: 2.5, 5 or 10% of pig pulmonary extract (Treatment group). As indicators of the treatment effect on semen quality, the live-dead ratio, the host test and the acrosome integrity were determined. Samples were evaluated after 0, 24 and 48 hours of incubation at 17 ° C and 36 ° C. The experiment was repeated in three ejaculates of each one of the boars, in order to determine interaction between the components of the preservation medium and the pulmonary extracts, and the quality of the porcine sperm. The results show that there are no differences in the parameters observed between the different evaluation moments, nor in the different extract variations, as well as no difference between individuals. It is concluded that pulmonary extracts do not present toxic effects on porcine sperm.

Downloads

Download data is not yet available.

Author Biographies

Dante Alberto Cerutti, Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias

Médico Veterinario. Especialista en Reproducción Bovina. Magister en Reproducción. Cátedra de Reproducción Animal Facultad de Ciencias Veterinarias. UNLPam

Melina Castillo, Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias

Médica Veterinaria. Especialista en Sanidad de los Rumiantes domésticos. Cátedra de Biología General y Cátedra de Inmunología Especial.

Humberto Cisale, Universidad de Buenos Aires

Médico veterinario de la Universidad de Buenos Aires, doctorado en Biotecnología de la Reproducción Animal. Es profesor titular de Física Biológica en la Facultad de Veterinaria (UBA) y ex vicedecano de la misma facultad. Impartió cursos en la Universidad de León (España), el Colegio Oficial de Veterinarios de la Provincia de Zamora (España), la Sociedad Argentina de Biología, y la Universidad Nacional de La Plata. 

References

Graham E, Crabo B. Some Factors Influencing the Freezing Of Boar Spermatozoa.

Proc. 7th. Int. Cong. Anim. Reprod., München 1972, Vol. 2, pp. 1627.

Shulman V, Johnson I. Effect Of Orvus Es Paste On Acrosome Morphology, Motility

And Fertilizing Capacity Of Frozen-Thawed Boar Semen,Pursel. J. Anim. Sci. 1978. 47:

-202.

Martin J, Klug E, Günzel A. Investigations On Centrifugation Of Stallion Semen And

Storage In Large Volume Straws. 2nd. Int. Symposium on Equine Reproduction. 1978

Davis, California, 24-28.07.

Hellemann C, Gonzalez M, Guzman A. Efecto De Yema De Huevo en Polvo, un Surfactante y Centrifugación En La Sobrevivencia De Espermatozoides Caprinos Congelados, Arch. Med. Vet. 1992. 24(2): 23-30.

Arriola J, Foote H. Glycerolation And Thawing Effects On Bull Spermatozoa Frozen

In Detergent-Treated Egg Yolk And Whole Egg Extenders. J. Dairy Sci. 1987 70:

-1670.

Hellemann M, Jara T. Efecto De Un Surfactante Sobre La Integridad De Espermatozoides Ovinos Crioconservados, Centro de Inseminación Artificial, Universidad Austral de Chile. 1997. Casilla 567, Valdivia, Chile.

Alhaider A, Watson P. Cryopreservation Of Dog Semen: The Effects Of Equex Stm Paste On Plasma Membrane Fluidity And The Control Of Intracellular Free Calcium. Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street. 2009. London NW1 0TU, UK

Axnér E, Hermansson U, Linde-Forsberg C. The Effect Of Equex Stm Paste And Sperm

Morphology On Post-Thaw Survival Of Cat Epididymal Spermatozoa. Department of

Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of

Agricultural Sciences. 2004. P.O. Box 7039, S-75007 Uppsala, Sweden.

Marotta E. Extracción de semen en el cerdo: técnica y material. Revista de Medicina

Veterinaria 1973. 54:2534.

Published

2021-03-30

How to Cite

Cerutti, D. A., Castillo, M., Ramos, S., Koncurat, M. A., & Cisale, H. (2021). Determination of the Toxic Effect of Pulmonary Extracts on Refrigerated Porcine Semen. Ciencia Veterinaria, 23(1), 39–46. https://doi.org/10.19137/cienvet202123103

Issue

Section

Artículos de Investigación