Isolation and characterization of Brucella abortus biovar1 in aborted foetuses from cattle herds in the Province of Buenos Aires, Argentina
Isolation and characterization of Brucella abortus biovar1 in aborted foetuses from cattle herds in the Province of Buenos Aires, Argentina
DOI:
https://doi.org/10.19137/cienvet.v28.9805Keywords:
Brucella aborus, brucellosis, biovarAbstract
During 2025, the Azul Laboratory received a total of 68 aborted bovine foetuses from different herds in the Province of Buenos Aires for etiological diagnosis. In 7 of them, originated from the districts of Alvear, Lamadrid, Benito Juárez, Ayacucho, and Tapalqué, Brucella spp. was detected and isolated from abomasal fluid and lung samples. Initial detection was performed using direct immunofluorescence and Stamp staining, followed by culture on selective media. The isolates were sent to the Brucellosis Department of SENASA, where they were confirmed by biochemical tests and multiplex PCR as belonging to Brucella abortus biovar 1 field strains, ruling out the involvement of the vaccine strains S19 and RB51. These findings demonstrate the active circulation of B. abortus in breeding herds in the Pampas region, despite the implementation of the National Control and Eradication Plan
References
Organización Mundial de Sanidad Animal. (2024). Brucelosis (infección por Brucella abortus, B. melitensis y B. suis). En Manual de las Pruebas de Diagnóstico y de las Vacunas para los Animales Terrestres (Cap. 3.1.4). OMSA.
Pappas, G., Papadimitriou, P., Akritidis, N., Christou, L., & Tsianos, E. V. (2006). The new global map of human brucellosis. The Lancet. Infectious diseases, 6(2), 91–99. https://doi.org/10.1016/S1473-3099(06)70382-6
Corbel, M. J. (2006). Brucellosis in humans and animals. World Health Organization.
Seleem, M. N., Boyle, S. M., & Sriranganathan, N. (2010). Brucellosis: a re-emerging zoonosis. Veterinary microbiology, 140(3-4), 392–398. https://doi.org/10.1016/j.vetmic.2009.06.021
Gul H, Erdem H. Mandell, Douglas, and Bennett’s. 2020. Principles and Practice of Infectious Diseases. 9th ed. Elselvier.
Pereira, C. R., Cotrim de Almeida, J. V. F., Cardoso de Oliveira, I. R., Faria de Oliveira, L., Pereira, L. J., Zangerônimo, M. G., Lage, A. P., & Dorneles, E. M. S. (2020). Occupational exposure to Brucella spp.: A systematic review and meta-analysis. PLoS neglected tropical diseases, 14(5), e0008164. https://doi.org/10.1371/journal.pntd.0008164
Moreno, E., Blasco, J. M., & Moriyón, I. (2022). Facing the Human and Animal Brucellosis Conundrums: The Forgotten Lessons. Microorganisms, 10(5), 942. https://doi.org/10.3390/microorganisms10050942
Walker, L.R. (1999). Brucella. In: Hirsh, D.C. and Zee, Y.C., Eds., Veterinary Microbiology, Blackwell Science, USA, 196-202.
Ley Nº 24.557. (1995, 3 de octubre). Ley de Riesgos del Trabajo. Congreso de la Nación Argentina. Boletín Oficial de la República Argentina. infoleg.gob.ar
SENASA, 2019. Resolución 67/2019. SENASA, Argentina
Godfroid, J., Nielsen, K., & Saegerman, C. (2010). Diagnosis of brucellosis in livestock and wildlife. Croatian medical journal, 51(4), 296–305. https://doi.org/10.3325/cmj.2010.51.296
Alton G.G., Jones L.M., Angus R.D. & Verger J.M. (1988). Techniques for the Brucellosis Laboratory. Institut National de la Recherche Agronomique, Paris, France
Terzolo, H. R., Paolicchi, F. A., Moreira, A. R., & Homse, A. (1991). Skirrow agar for simultaneous isolation of Brucella and Campylobacter species. species. The Veterinary record, 129(24), 531–532.
López-Goñi, I., García-Yoldi, D., Marín, C. M., de Miguel, M. J., Muñoz, P. M., Blasco, J. M., Jacques, I., Grayon, M., Cloeckaert, A., Ferreira, A. C., Cardoso, R., Corrêa de Sá, M. I., Walravens, K., Albert, D., & Garin-Bastuji, B. (2008). Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains. Journal of clinical microbiology, 46(10), 3484–3487. https://doi.org/10.1128/JCM.00837-08
Hollender, D., Conde, S. B., Salustio, E., & Samartino, L. E. (2013). Detección de un complejo clonal con el genotipo de Brucella abortus biovariedad 2 como fundador en aislamientos de B. abortus de Argentina [Detection of a clonal complex with Brucella abortus biovar 2 genotype as founder in B. abortus isolates from Argentina]. Revista Argentina de microbiologia, 45(4), 229–239. https://doi.org/10.1016/S0325-7541(13)70029-4
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